作者musicman (AJ)
站內Biotech
標題[期刊] Design and Application of a shRNA-Based Gene Replacement
時間Sat Aug 21 13:05:54 2010
1. 這篇文章大概描述(
中文30字)....
Using virus, expression of the endogenous Anti-Silencing Function
1a (ASF1a) histone chaperone has been efficiently replaced in primary
human cells, by an ectopically expressed epitope-tagged version.
Moreover, the virus is designed so that other shRNA and shRNA-resistant
cDNA cassettes can easily be substituted, making the approach readily
applicable to other protein targets.
[
期刊名稱 與 標題卷號年份等資訊]
1.Methods in Molecular Biology, 2007, Volume 408, III, 211-221,
DOI: 10.1007/978-1-59745-547-3_12
[
期刊網址/ 太長的話 請到
http://tinyurl.com縮網址]
1.
http://tinyurl.com/2ekkjrx
[
我的e-mail]
[email protected]
跪求各位先進~感恩~~
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1F:推 IL12:好沒誠意呀,簡介直接剪貼 ... 08/21 16:32
2F:→ musicman:SORRY~由於剛進博班新實驗室 領域一竅不通 老闆突然要我 08/21 17:55
3F:→ musicman:幫他找這篇...一時也不知道怎麼說明~ 08/21 17:55
4F:→ ordinary07:基本上就我的瞭解 這期刊比較像一本書 不會有單獨的pdf 08/21 20:07
5F:→ ordinary07:如果想拿到 其實你可以拿去 google 看看運氣和耐心了.. 08/21 20:12
6F:→ ordinary07:不然就去 nature protocl 找篇類似的給老師吧... 08/21 20:13
7F:→ musicman:好的 感謝樓上O大~!! 08/21 21:02
8F:→ aceliang:Methods in molecular biology精裝版各大學館藏較多。 08/21 22:22
9F:→ ranilla:這篇文章是用針對UTR的序列來設計shRNA另外再加入CDS的 08/21 22:45
10F:→ ranilla:OVEREXPRESSION在同一個質體內 08/21 22:46
11F:→ ranilla:下不為例喔,因為有違板規,希望你看完後,可以改一改 08/21 22:47
12F:→ ranilla:done~ 08/21 22:47
13F:→ musicman:ORZ 感謝ran大!!!! 08/21 22:52