作者musicman (AJ)
站内Biotech
标题[期刊] Design and Application of a shRNA-Based Gene Replacement
时间Sat Aug 21 13:05:54 2010
1. 这篇文章大概描述(
中文30字)....
Using virus, expression of the endogenous Anti-Silencing Function
1a (ASF1a) histone chaperone has been efficiently replaced in primary
human cells, by an ectopically expressed epitope-tagged version.
Moreover, the virus is designed so that other shRNA and shRNA-resistant
cDNA cassettes can easily be substituted, making the approach readily
applicable to other protein targets.
[
期刊名称 与 标题卷号年份等资讯]
1.Methods in Molecular Biology, 2007, Volume 408, III, 211-221,
DOI: 10.1007/978-1-59745-547-3_12
[
期刊网址/ 太长的话 请到
http://tinyurl.com缩网址]
1.
http://tinyurl.com/2ekkjrx
[
我的e-mail]
[email protected]
跪求各位先进~感恩~~
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◆ From: 140.120.208.42
1F：推 IL12:好没诚意呀，简介直接剪贴 ... 08/21 16:32
2F：→ musicman:SORRY~由於刚进博班新实验室 领域一窍不通 老板突然要我 08/21 17:55
3F：→ musicman:帮他找这篇...一时也不知道怎麽说明~ 08/21 17:55
4F：→ ordinary07:基本上就我的了解 这期刊比较像一本书 不会有单独的pdf 08/21 20:07
5F：→ ordinary07:如果想拿到 其实你可以拿去 google 看看运气和耐心了.. 08/21 20:12
6F：→ ordinary07:不然就去 nature protocl 找篇类似的给老师吧... 08/21 20:13
7F：→ musicman:好的 感谢楼上O大~!! 08/21 21:02
8F：→ aceliang:Methods in molecular biology精装版各大学馆藏较多。 08/21 22:22
9F：→ ranilla:这篇文章是用针对UTR的序列来设计shRNA另外再加入CDS的 08/21 22:45
10F：→ ranilla:OVEREXPRESSION在同一个质体内 08/21 22:46
11F：→ ranilla:下不为例喔，因为有违板规，希望你看完後，可以改一改 08/21 22:47
12F：→ ranilla:done~ 08/21 22:47
13F：→ musicman:ORZ 感谢ran大!!!! 08/21 22:52