作者coolmanred (coolmanred)
看板Biotech
標題[求救] 請問測PKC-zeta assay
時間Tue Nov 11 00:50:46 2008
請問在測PKC activity時
利用免沉澱法 加入 MBP(myelin basic protein)的目的是什麼?
The immunoprecipitates were incubated with
2 μg MBP, 10 μCi [γ-32P]–ATP, 100 μM ATP for 30 min at 30°C.
The proteins were separated by SDS-PAGE, and radiolabeled MBP was
detected by autoradiography.
謝謝
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1F：推 wangba:MBP是PKC的substrate,藉由label的isotope可以測PKCactivity 11/11 01:20
2F：→ coolmanred:謝謝囉 11/11 21:47