作者coolmanred (coolmanred)
看板Biotech
标题[求救] 请问测PKC-zeta assay
时间Tue Nov 11 00:50:46 2008
请问在测PKC activity时
利用免沉淀法 加入 MBP(myelin basic protein)的目的是什麽?
The immunoprecipitates were incubated with
2 μg MBP, 10 μCi [γ-32P]–ATP, 100 μM ATP for 30 min at 30°C.
The proteins were separated by SDS-PAGE, and radiolabeled MBP was
detected by autoradiography.
谢谢
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1F：推 wangba:MBP是PKC的substrate,藉由label的isotope可以测PKCactivity 11/11 01:20
2F：→ coolmanred:谢谢罗 11/11 21:47