作者steffanie (Para Paradise)
看板Biotech
標題[求救] 幾提生化問題
時間Mon Oct 27 23:56:35 2008
1.(a) In gel filtration, larger proteins run faster or slower? Why?
我知道大的跑比較快 但不知道為什麼<囧
(b) In SDS-PAGE, larger proteins run faster or slower? Why?
我知道大的跑比較慢 但不知道為什麼<囧
2.(1) Can an antibody and its antigen be co-crystallized for X-ray
determination to study the interaction between this antibody
and its antigen? Why?
(2) Can we apply the same idea to study the interaction between
an enzyme and its allosteric effector or substrate individually
via co-crystallization? Why?
3.(a) Why do we add PITC in the analyses of amino acid composition
and sequencing?
(b) Why do we add 6 M HCl in the analysis of amino acid composition
but not in the analysis of amino acid sequencing?
(c) The existence of disulfide bonds in a protein blocks the Edman
reaction. How to solve this problem during amino acid sequencing?
請高手指點迷津 感激不盡:)
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◆ From: 61.58.155.102
1F：推 bonbonyaho:認真一點查查書...加油，好嗎? 10/27 23:59
2F：推 sharkcell:這...應該很課本吧...超生化的題目，原po加油!! 10/28 02:10
3F：→ sharkcell:離開課本太久了沒辦法給建議Orz 10/28 02:10
4F：推 zoom8080:回答第一提 膠體純化小分子會進去樹脂裡鑽一鑽再出來 10/29 01:07
5F：→ zoom8080:大分子直接通過樹脂顆粒之間 所以先出來 10/29 01:08
6F：→ zoom8080:SDS膠裡面很多小洞，小分子走的快 大分子比較擠 走得慢 10/29 01:09
※ 編輯: steffanie 來自: 61.58.155.102 (10/30 22:17)