作者steffanie (Para Paradise)
看板Biotech
标题[求救] 几提生化问题
时间Mon Oct 27 23:56:35 2008
1.(a) In gel filtration, larger proteins run faster or slower? Why?
我知道大的跑比较快 但不知道为什麽<囧
(b) In SDS-PAGE, larger proteins run faster or slower? Why?
我知道大的跑比较慢 但不知道为什麽<囧
2.(1) Can an antibody and its antigen be co-crystallized for X-ray
determination to study the interaction between this antibody
and its antigen? Why?
(2) Can we apply the same idea to study the interaction between
an enzyme and its allosteric effector or substrate individually
via co-crystallization? Why?
3.(a) Why do we add PITC in the analyses of amino acid composition
and sequencing?
(b) Why do we add 6 M HCl in the analysis of amino acid composition
but not in the analysis of amino acid sequencing?
(c) The existence of disulfide bonds in a protein blocks the Edman
reaction. How to solve this problem during amino acid sequencing?
请高手指点迷津 感激不尽:)
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◆ From: 61.58.155.102
1F：推 bonbonyaho:认真一点查查书...加油，好吗? 10/27 23:59
2F：推 sharkcell:这...应该很课本吧...超生化的题目，原po加油!! 10/28 02:10
3F：→ sharkcell:离开课本太久了没办法给建议Orz 10/28 02:10
4F：推 zoom8080:回答第一提 胶体纯化小分子会进去树脂里钻一钻再出来 10/29 01:07
5F：→ zoom8080:大分子直接通过树脂颗粒之间 所以先出来 10/29 01:08
6F：→ zoom8080:SDS胶里面很多小洞，小分子走的快 大分子比较挤 走得慢 10/29 01:09
※ 编辑: steffanie 来自: 61.58.155.102 (10/30 22:17)