作者Nikio (我要出國!)
看板Biotech
標題Re: [討論] 有關免疫的問題...
時間Sat Oct 25 17:38:45 2008
※ 引述《nidus (咕嘰)》之銘言：
: PAPER出處：
: http://www.cell.com/content/article/abstract?uid=PIIS0092867408008210
: 這篇作者利用 IL2 receptor gamma knockout NOD/SCID 為model
: 使靜脈注射到老鼠體內的human peripheral blood leukocytes(PBL)
: 不會受到小鼠免疫排斥
: 但是人類的leukocytes到小鼠體內
: 難道不會對小鼠體內的各種組織器官進行免疫反應嗎
: 煩請好心的大大為不才解困 謝謝^^
不會反應，他實驗方法也不是用i.v打
Hu-HSC 打的是幹細胞到胎鼠，被ANERGY掉或者被positive及negative selection後存活
因此不可能反應
Hu-HSC mice were generated as described (Ishikawa et al., 2005).
One- to two-day-old neonatal mice were irradiated (100 rads) and injected
iv with T cell-depleted cord blood cells containing 3 × 104 CD34+ cells
per mouse. Transplanted mice were tested for engraftment 12 weeks later
as described above
Hu-PBL mice有人證明過i.p打到老鼠內1~2月內都還是anergic狀態
（用i.p打造成，原因不明，但實驗證實之方法）
Recently, M. Tary-Lehmann and colleagues suggested that a
human immune response occurs in hu-PBL-SCID mice within
the first 2 to 3 weeks following PBL injection (31). Human
lymphoid cells residing in these mice are mature (mainly CD41
and CD81 cells), but the majority of these cells stain for
CD45RO and behave as anergic cells 1 to 2 months after i.p.
transplantation (29, 30).
http://jvi.asm.org/cgi/reprint/70/11/7958
但老鼠直接i.v打人類的PBL就鐵定會有排斥反應了
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1F：推 saviora:不過他同時有用這兩個model 10/25 21:20
2F：→ saviora:不過Hu-HSC那個的確不錯 10/25 21:21