作者Nikio (我要出国!)
看板Biotech
标题Re: [讨论] 有关免疫的问题...
时间Sat Oct 25 17:38:45 2008
※ 引述《nidus (咕叽)》之铭言：
: PAPER出处：
: http://www.cell.com/content/article/abstract?uid=PIIS0092867408008210
: 这篇作者利用 IL2 receptor gamma knockout NOD/SCID 为model
: 使静脉注射到老鼠体内的human peripheral blood leukocytes(PBL)
: 不会受到小鼠免疫排斥
: 但是人类的leukocytes到小鼠体内
: 难道不会对小鼠体内的各种组织器官进行免疫反应吗
: 烦请好心的大大为不才解困 谢谢^^
不会反应，他实验方法也不是用i.v打
Hu-HSC 打的是干细胞到胎鼠，被ANERGY掉或者被positive及negative selection後存活
因此不可能反应
Hu-HSC mice were generated as described (Ishikawa et al., 2005).
One- to two-day-old neonatal mice were irradiated (100 rads) and injected
iv with T cell-depleted cord blood cells containing 3 × 104 CD34+ cells
per mouse. Transplanted mice were tested for engraftment 12 weeks later
as described above
Hu-PBL mice有人证明过i.p打到老鼠内1~2月内都还是anergic状态
（用i.p打造成，原因不明，但实验证实之方法）
Recently, M. Tary-Lehmann and colleagues suggested that a
human immune response occurs in hu-PBL-SCID mice within
the first 2 to 3 weeks following PBL injection (31). Human
lymphoid cells residing in these mice are mature (mainly CD41
and CD81 cells), but the majority of these cells stain for
CD45RO and behave as anergic cells 1 to 2 months after i.p.
transplantation (29, 30).
http://jvi.asm.org/cgi/reprint/70/11/7958
但老鼠直接i.v打人类的PBL就铁定会有排斥反应了
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1F：推 saviora:不过他同时有用这两个model 10/25 21:20
2F：→ saviora:不过Hu-HSC那个的确不错 10/25 21:21