Hock-immunization protocol For hock immunization in the left hind foot, the mouse is placed on the rack underneath the cage top and held gently by just the left foot with the top down. To do the hock injection with a CFA emulsion, a right-handed person wo uld hold the left hind foot between the left thumb and left middle finger le aving the left index finger free. Holding the loaded syringe and needle read y in the right hand, inject laterally on the right side (inside part of the leg) just above the ankle into the space indicated in Fig. 1. For footpad immunization in the left hind foot, the mouse is placed on the r ack underneath the cage top and held gently by just the left foot with the t op down and the ventral part of the foot facing up. For the injections, a 27-gauge needle was used and the injection site approa ched with the bevelled edge of the needle facing to the left in the case of hock immunization, and downwards in the case of footpad immunization, i.e. t he bevel facing the skin while going in with the needle. The needle was push ed into the skin a little way past the bevel before starting to inject. Afte r finishing to inject, the needle was slowly withdrawn from the injection si te while pressing down lightly on top of the injection site with the left in dex finger. Once the needle was completely withdrawn from the injection site , it was pressed down a little harder for a few seconds, maybe 2 to 3 second s, and then released. At this point, there should be no injected material oo zing from the site of the injection. If material was still oozing out, the i njection site was pressed down for a few seconds more 大家好我想请问有人打过这种打老鼠的注射方法吗 我是硕士班学生目前实验室没人会 我也还没会><试过很多次都没成功 我的理解是他要打进去老鼠的脚踝上方 可是我不知道改打进哪里?我的问题是要打进血管吗?还是那个淋巴管?还是皮下? 网路上也查不到啥麽资料.. 想问问前辈们谢谢https://i.imgur.com/vjgfir9.jpg --

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