作者enisx (浪费眼力...)
看板Biotech
标题Re: [问题] do I have to sterilize TE buffer?
时间Wed Mar 28 11:22:50 2007
※ 引述《YuDavid (讨厌失眠)》之铭言：
: Excuse me, I'm now having a bacteria total DNA extraction experiment,
: which need of using TE buffer (10mM Tris-HCl, 0.1mM EDTA).
: i was just wondering do i have to sterilze TE buffer before using it?
: And is this materail capable to sterilize?
: Tanks for those who help me!^^
Autoclave: most buffers and salt solutions
bacterial and yeast media
Do not autoclave:
buffers with detergents (SDS)
organic solvents (ethanol, acetone, phenol, chloroform)
heat labile ingredients (vitamins, hormones, antibiotics,
proteins)
HEPES-containing solutions
DTT- (dithiothreitol) or BME- (beta-mercaptoethanol)
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1F：推 aggaci:为什麽HEPES containing solution不行哩？ 03/28 11:31
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3F：推 naturer:urea会沈淀.....orz 03/28 19:03