作者r07h41013 (Jack)
看板translator
標題[筆譯]學術論文英翻中_2元/字_7485字
時間Wed Apr 17 09:58:21 2019
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[必]工 作 量：7485英字(確認接案會請譯者一起核對字數)
[必]工作報酬：15000
[必]涉及語言：英譯中
[必]所屬領域：生物、醫學
[必]文件類型：論文(33頁)、期刊(4頁)
[必]截 稿 日：徵到後5日
[必]應徵期限：徵到為止
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[選]工作要求：詳見注意事項。
[選]參考段落：見試譯文。
[選]試 譯 文：
(2) Inhibition of fat accumulation
a. Suppresses differentiation of 3T3-L1 adipocytes (in vitro)
Experimental design and treatment:
3T3-L1 adipocytes (5x104 cells/ml) were cul
tured in DMEM medium (high glucose)
supplemented with 10% fetal calf serum. Ce
lls differentiation was induced by medium replacement containing
insulin (1 μg/ml), dexamethasone (0.25
μM) and isobutyl-methyxanthine (0.5 mM). 2 days later, the medium
containing Coffee Bean Extract and its
functional components and insulin (1μg/ml) was introduced.
The new medium was cultured for 6 days
with replacement every alternate day. Differentiation of adipocytes
and cell triglyceride levels were dete
rmined. Cell differentiation was determined using GPDH
(glycerol 3-phosphate dehydrogenase) as biochemical marker.
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※ 編輯: r07h41013 (140.112.25.33), 04/17/2019 10:00:42
※ 編輯: r07h41013 (49.216.0.130), 04/17/2019 13:49:06