一. 九大題問答, 給之後要考陽明生化的人參考囉^^ (其他所的生化有些也是考這份題目) 二. 本文歡迎轉載, 但請補習班勿用於商業用途!or2 (要賣考古的話請派槍手去抄出來XD) 1. We consume food to provide energy for the body, but the majority of the energy in food is not in the form of ATP. How does the body utilize energy from other nutrients in the diet to produce ATP?? (10 points) 2. Please explain why electrons travel from NADH-Q reductase, to ubiquinone(Q), to cytochrome reductase, rather than in the opposite direction?? (10 points) 3. Cholesterol in human can be obtained from the diet or synthesized de novo. An adult human on a low-cholesterol diet typically synthesized 600 mg of cholesterol per day in the liver. If the amount of cholesterol in the diet is large, de novo synthesis of cholesterol is drastically reduced. How is the regulation brought about?? (10 points) 4. Please describe what you learn form the following table. (10 points) | 10℃ | 20℃ | 30℃ | 40℃ | -------------------------------------------------------------------- Myristic acid (14:0) | 4 | 4 | 4 | 8 | Percantage Palmitic acid (16:0) | 18 | 25 | 29 | 48 | of Palmitoleic acid (16:1) | 26 | 24 | 23 | 9 | total Oleic acid (18:1) | 38 | 34 | 30 | 12 | fatty Hydroxymyristic acid | 13 | 10 | 10 | 8 | acids -------------------------------------------------------------------- Ratio of unsaturated | 2.9 | 2.0 | 1.6 | 0.38 | to saturated | | | | | -------------------------------------------------------------------- 5. Consider the effects of two enzymes. Enzyme A catalyzed the reaction: ATP + GDP <=>(上下雙箭號XD) ADP + GTP, whereas enzyme B catalyzed the reaction: NADH + NADP+(上標) <=> NAD+ + NADPH Discuss whether the enzymes would be beneficial or dertrimetal to cells. 6. (A) What is the RNA interference(RNAi)?? (3 points) (B) Please describe the gene knockdown mechanisms of RNAi?? (12 points) 7. (A) What are the differences between DNA and RNA?? (5 points) (B) You have a test tube containing nucleic acids in buffer. How do you tell whether it is DNA or RNA?? (C) You have a test tube containing cell lysate isolated form HeLa cells. How do you remove proteins, purify nucleic acids and isolated mRNA from cell lysate?? (Do Not Use Commerical Kits!!) 8. A peptide has the sequence "KQEIFNYTW". What is the mRNA sequence?? (有附表-[The genetic code.]) 9. You did a co-immunoprecipitation experiment and found that protein A was co-immunoprecipated with protein B. According to this result, can you conclude that protein A directly interacts with protein B?? Why?? What will you do to further confirm this conclusion?? (10 points) -- ~~ 讀者猛地里一躍而起，奔到漫畫店前，瞧著火鳳黑頁裡的那幾行字， 　 大聲叫道”十六週後，復刊相會，精采連載，勿失信約” 　 陳某阿陳某，是你親手畫下的黑頁，怎地你不守信約？怎地你不守信約？ "休刊十六週了~ 為何還不復刊呀~~~~ 黑暗休法呀~~~~orz" --

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