一. 九大题问答, 给之後要考阳明生化的人参考罗^^ (其他所的生化有些也是考这份题目) 二. 本文欢迎转载, 但请补习班勿用於商业用途!or2 (要卖考古的话请派枪手去抄出来XD) 1. We consume food to provide energy for the body, but the majority of the energy in food is not in the form of ATP. How does the body utilize energy from other nutrients in the diet to produce ATP?? (10 points) 2. Please explain why electrons travel from NADH-Q reductase, to ubiquinone(Q), to cytochrome reductase, rather than in the opposite direction?? (10 points) 3. Cholesterol in human can be obtained from the diet or synthesized de novo. An adult human on a low-cholesterol diet typically synthesized 600 mg of cholesterol per day in the liver. If the amount of cholesterol in the diet is large, de novo synthesis of cholesterol is drastically reduced. How is the regulation brought about?? (10 points) 4. Please describe what you learn form the following table. (10 points) | 10℃ | 20℃ | 30℃ | 40℃ | -------------------------------------------------------------------- Myristic acid (14:0) | 4 | 4 | 4 | 8 | Percantage Palmitic acid (16:0) | 18 | 25 | 29 | 48 | of Palmitoleic acid (16:1) | 26 | 24 | 23 | 9 | total Oleic acid (18:1) | 38 | 34 | 30 | 12 | fatty Hydroxymyristic acid | 13 | 10 | 10 | 8 | acids -------------------------------------------------------------------- Ratio of unsaturated | 2.9 | 2.0 | 1.6 | 0.38 | to saturated | | | | | -------------------------------------------------------------------- 5. Consider the effects of two enzymes. Enzyme A catalyzed the reaction: ATP + GDP <=>(上下双箭号XD) ADP + GTP, whereas enzyme B catalyzed the reaction: NADH + NADP+(上标) <=> NAD+ + NADPH Discuss whether the enzymes would be beneficial or dertrimetal to cells. 6. (A) What is the RNA interference(RNAi)?? (3 points) (B) Please describe the gene knockdown mechanisms of RNAi?? (12 points) 7. (A) What are the differences between DNA and RNA?? (5 points) (B) You have a test tube containing nucleic acids in buffer. How do you tell whether it is DNA or RNA?? (C) You have a test tube containing cell lysate isolated form HeLa cells. How do you remove proteins, purify nucleic acids and isolated mRNA from cell lysate?? (Do Not Use Commerical Kits!!) 8. A peptide has the sequence "KQEIFNYTW". What is the mRNA sequence?? (有附表-[The genetic code.]) 9. You did a co-immunoprecipitation experiment and found that protein A was co-immunoprecipated with protein B. According to this result, can you conclude that protein A directly interacts with protein B?? Why?? What will you do to further confirm this conclusion?? (10 points) -- ~~ 读者猛地里一跃而起，奔到漫画店前，瞧着火凤黑页里的那几行字， 　 大声叫道”十六周後，复刊相会，精采连载，勿失信约” 　 陈某阿陈某，是你亲手画下的黑页，怎地你不守信约？怎地你不守信约？ "休刊十六周了~ 为何还不复刊呀~~~~ 黑暗休法呀~~~~orz" --

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