課程名稱︰蛋白質體學 課程性質︰選修 課程教師︰主授張英峰（此範圍為張英峰/鄭貽生老師共同授課） 開課學院：生命科學院 開課系所︰生命科學系 考試日期（年月日）︰2017/04/17 考試時限（分鐘）：120分鐘 考試範圍： Introduction (張英峰) From genome to proteome (鄭貽生) Sample preparation (張英峰) Protein separation and detection (張英峰) Protein digestion and peptide separation (張英峰) Mass spectrometry (張英峰) 試題 : A. Please choose one right answer for each questions: (15%) 1. What is the maximum miss cleavage number for the following peptide EKMSTELKEYRTVWYM if trypsin is used? (1) 0; (2)1; (3)2; (4)3; (5)4 2. If m/z value 1334.5 was observed using MALDI-TOF, what is the actual mass of the analyte? (1) 1334.5; (2) 1335.5; (3) 1333.5; (4) 2669; (5) 667.7 3. Which of the following does not aim at sub-proteome analysis? (1) Organelle isolation; (2) Affinity purification of protein complex; (3) Phosphopeptide enrichment; (4) Total protein isolation and analysis 4. What feature of peptides is used for C4 Zip-tip to clean up proteins? (1) charge; (2) mass; (3) hydrophobicity; (4) size 5. Which of the following does not have tandem mass spectrometer? (1) Q-TOF; (2) TOF-TOF; (3) MALDI-TOF; (4) Triple-Q B. Please link the two: (10%) 1. ESI a. y. b ions 2. MALDI b. zwitterion 3. Ampholyte c. mutiple charged ions 4. CID d. cy3, cy5 5. DIGE e. singly charged ions 1-c, 2-e, 3-b, 4-a, 5-d C. Please define the following terms: (15%) 1. Edman degradation 2. Alpha-CHCA 3. ETD 4. CE-MS 5. Zwitterion D. Please explain "Why is Proteomics necessary for scientific research?" (14%) E. Please explain how NEphGE works in a proteomic study. (5%) F. Please explain how MALDI works in a mass spectrometer. (5%) G. Please write out the contribution of researchers John Fenn, Koichi Tanaka and John Yates respectively. (5%) H. Please describe how internal calibration is performed for MALDI-TOF analysis? (5%) I. Please describe how MudPIT works in a proteomic study. (10%) J. Please describe and compare different protein staining methods. (6%) K. What is the possible cleavage fragment for the peptide TGMHLETYKDERLSK if trypsin, V8, and CNBr are used individually? (10%) (PS. 本堂課無期末考，期末需繳交mini-review，期中考目的重在了解基本原理) --

※ 發信站: 批踢踢實業坊(ptt.cc), 來自: 140.112.25.105 ※ 文章網址: https://webptt.com/m.aspx?n=bbs/NTU-Exam/M.1492403860.A.5D1.html