课程名称︰蛋白质体学 课程性质︰选修 课程教师︰主授张英峰（此范围为张英峰/郑贻生老师共同授课） 开课学院：生命科学院 开课系所︰生命科学系 考试日期（年月日）︰2017/04/17 考试时限（分钟）：120分钟 考试范围： Introduction (张英峰) From genome to proteome (郑贻生) Sample preparation (张英峰) Protein separation and detection (张英峰) Protein digestion and peptide separation (张英峰) Mass spectrometry (张英峰) 试题 : A. Please choose one right answer for each questions: (15%) 1. What is the maximum miss cleavage number for the following peptide EKMSTELKEYRTVWYM if trypsin is used? (1) 0; (2)1; (3)2; (4)3; (5)4 2. If m/z value 1334.5 was observed using MALDI-TOF, what is the actual mass of the analyte? (1) 1334.5; (2) 1335.5; (3) 1333.5; (4) 2669; (5) 667.7 3. Which of the following does not aim at sub-proteome analysis? (1) Organelle isolation; (2) Affinity purification of protein complex; (3) Phosphopeptide enrichment; (4) Total protein isolation and analysis 4. What feature of peptides is used for C4 Zip-tip to clean up proteins? (1) charge; (2) mass; (3) hydrophobicity; (4) size 5. Which of the following does not have tandem mass spectrometer? (1) Q-TOF; (2) TOF-TOF; (3) MALDI-TOF; (4) Triple-Q B. Please link the two: (10%) 1. ESI a. y. b ions 2. MALDI b. zwitterion 3. Ampholyte c. mutiple charged ions 4. CID d. cy3, cy5 5. DIGE e. singly charged ions 1-c, 2-e, 3-b, 4-a, 5-d C. Please define the following terms: (15%) 1. Edman degradation 2. Alpha-CHCA 3. ETD 4. CE-MS 5. Zwitterion D. Please explain "Why is Proteomics necessary for scientific research?" (14%) E. Please explain how NEphGE works in a proteomic study. (5%) F. Please explain how MALDI works in a mass spectrometer. (5%) G. Please write out the contribution of researchers John Fenn, Koichi Tanaka and John Yates respectively. (5%) H. Please describe how internal calibration is performed for MALDI-TOF analysis? (5%) I. Please describe how MudPIT works in a proteomic study. (10%) J. Please describe and compare different protein staining methods. (6%) K. What is the possible cleavage fragment for the peptide TGMHLETYKDERLSK if trypsin, V8, and CNBr are used individually? (10%) (PS. 本堂课无期末考，期末需缴交mini-review，期中考目的重在了解基本原理) --

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