課程名稱︰訊息傳遞與疾病 課程性質︰選修 課程教師︰主授陳青周（此範圍為陳青周/蔡丰喬/李明學老師授課） 開課學院：醫學院 開課系所︰醫衛共同課程 考試日期（年月日）︰2017/04/14 考試時限（分鐘）：110分鐘 試題 : (以下敘述均非與原題目完全相同，僅就考試主題作最接近之題目描述) 陳青周老師part：佔20分 (範圍：Introduction: Basics of cell signaling) 問答題： Please describe the receptor tyrosine kinase pathway and its relation to cancer cells. (20%) (加分題的概念，有時間請盡量發揮) ---------- 蔡丰喬老師part：佔30分 (範圍：GPCR signaling pathways + Second messengers) 一、選擇題：2題，各2分，均是問下列何者敘述錯誤 1. 敘述錯誤為 "The biggest population in the GRAFS system of GPCR is the Frizzled GPCR." 2. 敘述錯誤為 "PKA can be inactivated by the combination of cGMP." 二、是非題：6題，各1分，題幹敘述正確的居多 ＊ 有一題考Cholera toxin的致病機轉，題幹敘述"Gαs protein持續活化造成efflux of chloride ions and leads to secretion of H2O into the intestinal lumen." 應該是正確的 (因為上課沒講很仔細) 三、簡答題：共20分 1. Please list three examples of second messengers that impress you the most. Why? (6%) 2. Please list three features of GPCR signaling pathway that impress you the most. Why? (6%) 3. Please list two regulators of GPCR signaling pathway that impress you the most. Why? (4%) 4. Please list two molecules in the Ca2+ signaling pathway that impress you the most. Why? (4%) ＊ 簡答題題目均附有以下三點說明： (The item and reason will be one score respectively.) (Please summarize your answers in two lines. The answers outside the two lines will not be scored.) (You can give any examples including but not limited to their structures, functions, regulations, possible therapies or related drugs) ---------- 李明學老師part：佔60分 (範圍：RTK + Ras proteins + MAPK pathway + oncogenes/TSGs) 簡答題： 1. Please list out at least four reasons that will make the receptor tyrosine kinase EGFR constitutively active. (8%) 2. What are the molecular mechanisms of Src activation? What is the Src mutation in virus that will cause the sarcoma? (8%) 3. What are the five members in the Ras family? (5%) 4. What are the molecular mechanisms from GDP-associated Ras to GTP-associated Ras? (5%) 5. Why does the mutation G12D in Ras make it become constitutively active? (5%) (考前提醒有提到mutation會造成影響，但上課+講義中找不到...Orz) 6. How does MEK1/2 activate ERK1/2? What are the two groups of proteins regulated by ERK1/2? (8%) (答案似乎是轉錄因子TCF與RSF，很容易忽視帶過的地方) 7. What is the function of the MAPK scaffold proteins? (7%) 8. What are the possible reasons for proto-oncogenes to become oncogenes? Please list out at least four possibilities. (8%) 9. If BRCA1 or BRCA2 gains loss-of-function mutations in both of its allele, is there any possible therapeutic strategy to target this kind of cancer cells? (6%) ---------- 以上總分共計110分 --

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