課程名稱︰遺傳學 課程性質︰必修 課程教師︰于宏燦 開課學院：生科院 開課系所︰生科系 考試日期（年月日）︰2013/3/25 考試時限（分鐘）：3hr 是否需發放獎勵金：是 （如未明確表示，則不予發放） 試題 : 此為共同授課 範圍為2~6章-于宏燦老師 20~21章-郭志鴻老師 于老師部分 60% I. Terminology(40pts) 1. Genetic recombination 2. Norm of reaction 3. Breed true 4. Submetacentric chromosome 5. AFLPs 6. Penetrance 7. Overdominance 8. Genomic imprinting 9. Complementation 10. Maternal effect 11. Barr body 12. Extranuclear inheritance 13. Non-parental type 14. Testcross 15. Genetic linkage map 16. Pseudoautosomal region 17. Heterogametic sex 18. Dosage compensation 19. Genetic markers 20. Reciprocal cross II. Essay questions(20pts) 1. Below is a metaphase of a cell division (想像有一個V和三個形狀不同的X在中央板上 姊妹染色體分離) 1.1 Is this mitosis or meiosis? 1.2 If this is meiosis then draw an image of mitotic metaphase of this cell. If this is mitosis then draw an image of meiotic metaphase of this cell. 1.3 Explain why you have your answers? 2. Some explorers brought back two plants that were discovered in a remote mysterious valley. In appearance, the two plants look alike and have bisexual flowers. However, one produces round seeds and the other square seeds. Acoording to the discoveres, the plants were abundance in the valley, and they saw much more plants that were round-seed-bearing than square-seed-bearing. How would you design experiments to study the genetic mechanism that determines the shape of seed? 郭老師部分(40%) 1.(2pt) The term "genome" refers to ________________. 2.(2pt) _____________ uses the unique banding patterns of a chromosome to determine the general location of a gene. 3.(4pt) ____________ relies on DNA cloning techniques to determine the location of genes; the distances are computed in _______________. 4.(2pt) DNA microarrays can be used to monitor gene expression at the level of ____________. (hint: DNA, RNA, or protein?) 5.(6pt) List three possible explanations on why the proteome of an organism may be larger than is genome. 6.(4pt) If two genes are derived from the same ancestral sequence, they are called _____________. If these two genes are found in two different species, they are further defined as _______________. 7.(6pt) Assuming that you are planning to clone a bacterial genome (~5.4Mb) into a fosmid library (average inser size = ~40kb), how many clones do you need to achieve an estimated coverage of 6-fold? 8.(6pt) Assuming that you are preparing a grant proposal to sequence the genome of a fungus. Given the information below, please estimate the sequencing cost of this project. a. The genome size is estimated to be ~20 Mb. b. Using the shotgun sequencing approach, the goal is to provide an estimated coverage of 8-fold for assembly. c. The sequencing facility charges ＄100 for each Sanger sequencing read. The average read length is 800 bp. 9.(8pt) Use a dot matrix to calculate the sequence identity(%) between "MNTKELWTEV" and "MNTREIWTEV". --

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