课程名称︰遗传学 课程性质︰必修 课程教师︰于宏灿 开课学院：生科院 开课系所︰生科系 考试日期（年月日）︰2013/3/25 考试时限（分钟）：3hr 是否需发放奖励金：是 （如未明确表示，则不予发放） 试题 : 此为共同授课 范围为2~6章-于宏灿老师 20~21章-郭志鸿老师 于老师部分 60% I. Terminology(40pts) 1. Genetic recombination 2. Norm of reaction 3. Breed true 4. Submetacentric chromosome 5. AFLPs 6. Penetrance 7. Overdominance 8. Genomic imprinting 9. Complementation 10. Maternal effect 11. Barr body 12. Extranuclear inheritance 13. Non-parental type 14. Testcross 15. Genetic linkage map 16. Pseudoautosomal region 17. Heterogametic sex 18. Dosage compensation 19. Genetic markers 20. Reciprocal cross II. Essay questions(20pts) 1. Below is a metaphase of a cell division (想像有一个V和三个形状不同的X在中央板上 姊妹染色体分离) 1.1 Is this mitosis or meiosis? 1.2 If this is meiosis then draw an image of mitotic metaphase of this cell. If this is mitosis then draw an image of meiotic metaphase of this cell. 1.3 Explain why you have your answers? 2. Some explorers brought back two plants that were discovered in a remote mysterious valley. In appearance, the two plants look alike and have bisexual flowers. However, one produces round seeds and the other square seeds. Acoording to the discoveres, the plants were abundance in the valley, and they saw much more plants that were round-seed-bearing than square-seed-bearing. How would you design experiments to study the genetic mechanism that determines the shape of seed? 郭老师部分(40%) 1.(2pt) The term "genome" refers to ________________. 2.(2pt) _____________ uses the unique banding patterns of a chromosome to determine the general location of a gene. 3.(4pt) ____________ relies on DNA cloning techniques to determine the location of genes; the distances are computed in _______________. 4.(2pt) DNA microarrays can be used to monitor gene expression at the level of ____________. (hint: DNA, RNA, or protein?) 5.(6pt) List three possible explanations on why the proteome of an organism may be larger than is genome. 6.(4pt) If two genes are derived from the same ancestral sequence, they are called _____________. If these two genes are found in two different species, they are further defined as _______________. 7.(6pt) Assuming that you are planning to clone a bacterial genome (~5.4Mb) into a fosmid library (average inser size = ~40kb), how many clones do you need to achieve an estimated coverage of 6-fold? 8.(6pt) Assuming that you are preparing a grant proposal to sequence the genome of a fungus. Given the information below, please estimate the sequencing cost of this project. a. The genome size is estimated to be ~20 Mb. b. Using the shotgun sequencing approach, the goal is to provide an estimated coverage of 8-fold for assembly. c. The sequencing facility charges ＄100 for each Sanger sequencing read. The average read length is 800 bp. 9.(8pt) Use a dot matrix to calculate the sequence identity(%) between "MNTKELWTEV" and "MNTREIWTEV". --

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