作者fishguitar (魚吉他)
看板NCKU-IMM101
標題[情報]老師們好性急! 12/14 PBL也出現了!!
時間Tue Nov 30 16:22:12 2010
Guidelines and assignments for Mol Biol class on Dec 14, 2010
Reading:
Single molecule-sensitive probes for imaging RNA in live cells.
(
http://imm.med.ncku.edu.tw/news.htm )
公告事項中 PBL課程資源兩個PDF檔為此篇paper及supplementary!
1.Grouping: IMM and MT students are divided into 3 groups each
(i.e., IMM1,IMM2, IMM3, MT1, MT2, and MT3)
2.Prepare ppt slides
(the whole group, each slides with names of th contributors)
for
no more than 25 min presentation time, turn in by e-mail to
both
Dr. Wang ([email protected]) in IMM and Dr. Young
(
[email protected]) in MT
no later than 4 pm on Monday (Dec. 13)
3.Problem based learning for each group:
Each group needs to search more information from the PUBMED to clarify
the assigned problems/topics in the list below.
Please simplify complicated background with cartoons and some text explanation
If possible, you may use the following outlines to describe the
figures of the paper in details:
1. Background and Aim
2. Experimental strategy
3. How to prepare the experimental materials
4. Instruments (optional: available in NCKU ?) and methods
5. Results
6. Conclusion and impact
IMM1
Q1. Describe the key concepts involved in MTRIP methodologies and the
optimization strategies (Fig 1. a-c). Compare the difference between
MTRIPs and MS2-GFP binding systems in detection of intracellular
RNA molecules.
MT1
Q2. Please describe MTRIP sensitivity with experimental model on glass surfaces
and in the cytosol.
Please interpret the results in Fig 1. d, e and suppl Fig.1.
IMM2
Q3. Please describe the types (or composition) and functions of “cytoplasmic
RNA granules” and how these mRNA-protein organizations (mRNPs)
regulate mRNA.
MT2
Q4. How did the authors use MTRIP to visualize and quantify beta-actin mRNA in
A549 cells ? Please describe the instrument of time-lapse microscopy.
How did the authors record the tracking of beta-actin mRNA in A549 cells?
(Fig.a-d,suppl video 1-3) ?
IMM3
Q5. How did the authors use MTRIP to demonstrate (1) the co-localization of
beta-actin mRNA and its binding proteins (suppl Fig. 2-4);
and (2) the specifi detection of hRSV genome (suppl Fig. 5-7) .
Please explain in details how to correlate the imaged molecules
with their intensity and distribution (you may need to introduce
the Manders overlap coefficient and the Wilcoxon-Mann-Whitney test.)
MT3
Q6. How did the authors use MTRIP for the study of RNA-protein colocalizaiton
in live cells by using RSV RNA and TIA-1 in the absence and
presence of stress.
(Fig. 3a-d, suppl Fig 8, 9, suppl video 4-8).
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