作者oplz (Sometimes I Go Commando)
看板Medicine
標題Re: [STEM] 關於幹細胞的問題....完全不知道如何回答
時間Thu Jan 27 14:27:39 2011
※ 引述《ljii (是否)》之銘言：
: ※ 引述《huangtzuling (HTL)》之銘言：
: : 1 Discuss new strategies for using iPS cells in individual cell-based therapy
: 這可以天馬行空啊
: 變回iPS後可以重新引導分化成別的細胞,
: 所以可以拿來補已經死掉的身體組織
: 例如acute myocardial infarction的心肌細胞就是很好的選擇
: 目前現行醫療不能讓心肌再生,只能保住還沒死掉的細胞
: (但現在大家已經在作direct cardiomyocyte conversion了....早就跳過iPS了...)Orz)
personalized medicine.
iPS to beta cell to cure diabete.
(Rudy's lab @ MIT)
: : 2 Explain why haematopoietic stem cells are normally found in the bone
: : marrow of the adult
: 這...?? 因為骨髓的環境就是讓血球分化,成熟才放出來呀
: 我可能沒抓到題旨 這題是要問什麼?
Niche in bone marrow preventing it from fully differentiation.
: : 3 Discuss the technical and medical barriers to employing stem cells in
: : tissue engineering
: 1.要解決iPS需要開啟oncogene這回事
: 2.目前conversion factor都不成熟
: 要從iPS conversion成分化細胞 效率都沒有100%
: 例如80%好了 這20%的半成品是什麼?移植到人體內會不會有malignant potential?
: 沒人知道
1. low reprogramming efficiency, currently about or less than 1%.
Also, non-iPS cells will not be used on human body.
(eliminated through cell flow ans sorting)
2. Safety, old protocol using virus as vectors for high gene delivery
efficiency. New protocol using synthetic RNAs to solve this problem.
(Rossi Lab @ HMS)
3. epigentics, iPS cells still carry original epigenetic information after
reprogrammed. Thus, iPS cells from different tissues are still different
in some ways.
--
One out of four people in sciences are mentally unbalanced.
Think of three labmates.
If they seem okay, then you are the ONE.
--
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※ 編輯: oplz 來自: 134.174.140.32 (01/27 23:46)