作者oplz (Sometimes I Go Commando)
看板Medicine
标题Re: [STEM] 关於干细胞的问题....完全不知道如何回答
时间Thu Jan 27 14:27:39 2011
※ 引述《ljii (是否)》之铭言：
: ※ 引述《huangtzuling (HTL)》之铭言：
: : 1 Discuss new strategies for using iPS cells in individual cell-based therapy
: 这可以天马行空啊
: 变回iPS後可以重新引导分化成别的细胞,
: 所以可以拿来补已经死掉的身体组织
: 例如acute myocardial infarction的心肌细胞就是很好的选择
: 目前现行医疗不能让心肌再生,只能保住还没死掉的细胞
: (但现在大家已经在作direct cardiomyocyte conversion了....早就跳过iPS了...)Orz)
personalized medicine.
iPS to beta cell to cure diabete.
(Rudy's lab @ MIT)
: : 2 Explain why haematopoietic stem cells are normally found in the bone
: : marrow of the adult
: 这...?? 因为骨髓的环境就是让血球分化,成熟才放出来呀
: 我可能没抓到题旨 这题是要问什麽?
Niche in bone marrow preventing it from fully differentiation.
: : 3 Discuss the technical and medical barriers to employing stem cells in
: : tissue engineering
: 1.要解决iPS需要开启oncogene这回事
: 2.目前conversion factor都不成熟
: 要从iPS conversion成分化细胞 效率都没有100%
: 例如80%好了 这20%的半成品是什麽?移植到人体内会不会有malignant potential?
: 没人知道
1. low reprogramming efficiency, currently about or less than 1%.
Also, non-iPS cells will not be used on human body.
(eliminated through cell flow ans sorting)
2. Safety, old protocol using virus as vectors for high gene delivery
efficiency. New protocol using synthetic RNAs to solve this problem.
(Rossi Lab @ HMS)
3. epigentics, iPS cells still carry original epigenetic information after
reprogrammed. Thus, iPS cells from different tissues are still different
in some ways.
--
One out of four people in sciences are mentally unbalanced.
Think of three labmates.
If they seem okay, then you are the ONE.
--
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※ 编辑: oplz 来自: 134.174.140.32 (01/27 23:46)