3.In the lecture of Botanical Electron Microscopy, we have discussed a lot about three dimensional images of macromolecule and tissue. Could you correlate their instrumentation, method and application. The three dimensional appearance of SEM images is due to differences in contrast between various structural feature of the specimen when they are displayed on the viewing monitor. And the three dimensionality and contrast are due to the yield of secondary electrons from various parts of the specimen. Stereo imaging with the SEM Application: 1. The enhancement assists the viewer in discriminating between projections and depressions, and , it elucidates spatial relationship to help determine distances between two objects. Method: (generate stereo pairs and merge the separate views ) 1. Generate two different micrographs from separate viewpoint Generate stereo pairs a. shifting the specimen slightly to the left or right b. rotating the specimen c. deflecting the electron beam electronically d. tilting the electron beam electronically-------most prevalent example:(using tilt to generate stereo view in the SEM) 1.Take the first micrograph in the usual manner. 2.With out moving the specimen and trace the out line of the specimen on the CRT 3.Tilt the specimen slightly. 4.Refocus the specimen using the Z control adjustment of the stage. 5.Realign the image. 6.Adjust the brightness and contrast of the image in the SEM to match the first micrograph and take the second micrograph. 2. Merge these separate views using the appropriate tools (1)Print the two separate images at the same contrast and density levels in the dark room. (2)The micrographs are then placed alongside each other and viewed with a special set of glasses(stereo viewers) placed directly over the two prints. 我又來講解了 這個題目主要是要問說什麼是three dimensional image? 而我要如何去製作這個影像（method） 而他有什麼功能（application） 所用到的儀器（instrument） 我們主要是利用SEM image所產生的contrast，結合兩張影像利用人類的視差 （parallax phenomenon）使得本來是2D的照片看起來卻向是有3D的效果 而這主要的優點就是可以讓我們清楚的瞭解我們看到的影像中哪些是凸的哪些是凹的 並可以讓我們知道樣本之間的距離。 而如何去取得stereo pairs就是將specimen 稍微tilt一下取得兩張影像 在利用我們一般看立體圖的方法去觀察這樣就可以取得我們想要的3D立體圖了 或者是利用stereo viewer的幫忙。 -- 大家加油了剩下 最後一點點了 阿猛國偉趕快PO文呀 Orz -- --

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