作者super90579 (阿賢)
看板Grad-ProbAsk
標題[生醫] [生化] 關於合成DNA及Sanger定序法
時間Thu Feb 11 14:56:33 2010
最近做到兩題覺得不太懂得..
1.In DNA sequencing by the Sanger methed:
A)radioactive dideoxy ATP is included in each of four reaction mixtures
before enzymatic synthesis of complementary strands
B)以特殊酵素將新合成的DNA切成小片段，並跑電泳來分離。
C)the 雙去氧核苷酸 must be present at high levels to obtain long stretch
of DNA sequence.
D)the role of the dideoxy CTP is to occasionally terminate enzymatic
synthesis of DNA where Gs occur in the template strands.
E)the template DNA strand is radioactive.
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2.在化學合成DNA中
A)the dimethoxytrityl(DMT) group 會催化磷酸雙酯鍵的形成
B)合成的方向為5'向3'
C)合成oligonucleotide可以達到的最大長度為8-10個nucleotide.
D)the nucleotide initially attached to the silica gel support will become
the 3'end of the finished product.
E)the protecting cyanoethyl groups are removed after each step.
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第一題小弟選D
而第二題就沒有頭緒了..
小弟翻書也找不到在哪裡有
敢問一下是在哪個單元提到的..?
謝謝!!
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