作者super90579 (阿贤)
看板Grad-ProbAsk
标题[生医] [生化] 关於合成DNA及Sanger定序法
时间Thu Feb 11 14:56:33 2010
最近做到两题觉得不太懂得..
1.In DNA sequencing by the Sanger methed:
A)radioactive dideoxy ATP is included in each of four reaction mixtures
before enzymatic synthesis of complementary strands
B)以特殊酵素将新合成的DNA切成小片段，并跑电泳来分离。
C)the 双去氧核苷酸 must be present at high levels to obtain long stretch
of DNA sequence.
D)the role of the dideoxy CTP is to occasionally terminate enzymatic
synthesis of DNA where Gs occur in the template strands.
E)the template DNA strand is radioactive.
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2.在化学合成DNA中
A)the dimethoxytrityl(DMT) group 会催化磷酸双酯键的形成
B)合成的方向为5'向3'
C)合成oligonucleotide可以达到的最大长度为8-10个nucleotide.
D)the nucleotide initially attached to the silica gel support will become
the 3'end of the finished product.
E)the protecting cyanoethyl groups are removed after each step.
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第一题小弟选D
而第二题就没有头绪了..
小弟翻书也找不到在哪里有
敢问一下是在哪个单元提到的..?
谢谢!!
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