http://www.ambion.com/techlib/tb/tb_178.html 滅菌還是會有效果的 塑料器具要用NaOH處理 配藥的水要用DEPC處理過後滅菌 實驗區域盡量少有人走動 滅菌鍋也要記得清理和檢查 -- --

※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 211.76.175.171 1. Autoclaving is not effective at eliminating RNase in solution because the RNases simply renature as the solution cools. "FALSE", but... Autoclaving alone does indeed inactivate a substantial amount of RNase A (Figure 1). Various concentrations of RNase A were added to PBS and autoclaved. Aliquots of each solution were mixed with a 304 base 32P-labeled RNA probe and incubated at 37°C for one hour, followed by electrophoresis and exposure to film. Without autoclaving, the probe begins to degrade at an RNase concentration of 100 pg/ml. Autoclaving inactivates enough of the RNase A to protect the probe from degradation up to a concentration of 1 μg/ml. Note that only a portion of the RNase is inactivated by autoclaving, otherwise the RNA probe would remain intact at any RNase concentration. Autoclaving alone may be sufficient to eliminate enough RNase for some applications. However, since neither the extent of RNase contamination nor at what RNase concentration the assay is sensitive is known, DEPC should be used as an added precaution. Also note that these experiments were only performed on RNase A and may not hold true for other RNases. ※ 編輯: saviora 來自: 124.11.149.96 (09/10 23:54) 不好意思 ※ 編輯: saviora 來自: 124.11.149.96 (09/11 01:20)