我第一次要買細胞株 老師叫我去ATCC上看 我要買的是CHO CELL 但是一進去有37種的CHO 真的搞得我霧煞煞 裡面有些細胞株提到 This line is Neomycin resistant. The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC -HB-11081). The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences. The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins. 由於我即將要Transfect的plasmid 沒有細胞可辨識的selective gene 老師說 我可以考慮試試看cotransfection for stable cell line 以下是我的解讀 請大家指正 是指說這些細胞株本身就有可以抑制neomycin的功能 我若將我自己的plasmid用共轉染的方式放入cell中 可以做stable transfection 而不是只能做transient transfection 是這個意思嗎? 請大家幫幫忙 我該如何選擇細胞株呢? --

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