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標 題Re: [問題] Cloning:Enzyme digestion check
發信站清華生命科學 BBS (Wed Jul 4 20:20:32 2007)
轉信站ptt!Group.NCTU!grouppost!Group.NCTU!nthuls
※ 引述《[email protected] (Live.myself)》之銘言：
> ※ 引述《[email protected] (JUAN)》之銘言：
> : It depends on how the "enzyme check" experiment is designed.
> The vector is pET24a+,for 6xHis-tag fusion protein
> The empty vector is about 5-6kb
> The cutting sites selected are BamHI and XhoI
> Thanks again ^^
Normally, you should be able to see the insert fragment.
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