课程名称︰细胞生物学概论 课程性质︰选修 课程教师︰戴桓青 开课学院：理学院 开课系所︰化学系 考试日期（年月日）︰103/06/17 考试时限（分钟）：110分钟 是否需发放奖励金：是 （如未明确表示，则不予发放） 试题 : Intro cell Biology 2014 [Final Exam] Answer briefly in Chinese or English, 77pts total Q1. Membrane trafficking (12 pts) 　 　　Consider the following organelles: (A) late endosome (B) Golgi (C) endoplasmic reticulum (D) early endosome (E)lysosome (F) secretory vesicle 1-1. To secrete a protein such as immunoglobulin, three organelles are involved, please list them from early to late participation. 3 pts 1-2. To endocytose a G prtein-coupled receptor for degradation, three organelles are involved, please list them from ealy to late participation. 3 pts 1-3. Which organelles(s) have enzymes that add cabohydrates to protein? 4 pts 1-4. Which organelle acts like "thin layer chromatography" to separate membrane proteins and lipids? 2 pts Q2. Cytoskeleton (15 pts) 2-1. Please match the following proteins with different types of cytoskeleton. 6 pts (A) kinesin; (B) beta tubulin; (C) myosin; (D) vimentin; (E) keratin; (F) actin (X) microfilament; (Y) intermediate filament; (Z) microtubule 2-2. From A to F, which two are motor proteins? 2 pts 2-3. Microtubules and microfilaments have a plus end and a minus end. Which end is polymerizing more and which end is depolymerizing more? 2 pts 2-4. Please explain the treadmilling motion of actin and the role of ATP in treadmilling (draw a picture if necessary). 5 pts Q3. Protein degradation (16 pts) 3-1. There are hundred of proteases in the cell. Some of them are involved in posttranslational modification by cleaving a protein substrate. Please explain how this type of PTM is different from pretein degradation. 4 pts 3-2. How does the cell prevent accidental protein degradation by the lysosome? 4 pts 3-3. Proteasome is an enzyme complex. Lysosome is an organelle. Both are responsible for protein degradation. Which one is larger in terms physical dimensions? 2 pts 3-4. What is ubiquitin, ubiquitination, and the function of polyubiquitination? 6 pts Q4. Cell signaling (17 pts) 4-1. There are many messenger molecules inside and outside the cell, such as: (A) calcium; (B) insulin; (C) steroid hormones; (D) IP3 (inositol trisphosphate); (E) DAG (diacylglycerol); (F) cyclic GMP; (G) eicosanoids Which of the above are intracellular second messengers and which are excellular signals? 7 pts 4-2. Match Q to Z in the following picture (10 items) with the following terms (A to J). 10 pts (A) PIP2, phosphatidylinositol 4,5-bisphosphate (B) IP3, inositol trisphosphate (C) diacyglycerol (D) G protein α subunit (E) G protein β subunit (F) G protein γ subunit (G) G prtein-coupled receptor (H) phospolipase C (I) ligand (J) IP3 receptor Q5. Cancer (17 pts) 5-1. Please explain why cancer is a genomic disease. 4 pts 5-2. What is the minimum number of cancer cells that is sufficient to Cause a solid tumor? 2 pts 5-3. What is the most important tumor suppressor gene in the human genome (often mutated in cancers)? What is its normal function? 4 pts 5-4. Please explain the relationship between proto-oncegene and oncegene. 4 pts 5-5. Please explain how cancer immunotherapy works. 3 pts [Keep this question sheet, answer keys will be posted on CEIBA tomorrow] --

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