课程名称︰应用昆虫学 课程性质︰昆虫系大二必修 课程教师︰许如君/黄荣南/蔡志伟 合开 开课学院：生农学院 开课系所︰昆虫系 考试日期（年月日）︰99.06.22 考试时限（分钟）：100分钟 是否需发放奖励金：是 （如未明确表示，则不予发放） 试题 : 1.应用细胞或组织培养技术进行研究有何优缺点(10%) 2.下面一段摘要来自我们课堂上讲解过的一篇文章(A mechanism of cell death involving as adenylyl cyclase / PKA signaling pathway is indused by the Cry1Ab toxin of Bacillus thuringiensis),请把它翻成中文(15%) Many pathogenic organisms and their toxins target host cell receptors, the consequence of which is altered signaling events that lead to aberrant activity or cell death. A significant body of literature describes various molecular and cellular aspects of toxins associated with bacterial invasion, colonization, and host cell disruption. However, there is little information on the molecular and cellular mechanisms associated with the insecticidal action of Bacillus thuringiensis (Bt) Cry toxins. Recently,wereported that the Cry1Ab toxin produced by Bt kills insect cells by activating a -dependent cytotoxic event upon binding of the toxin to its receptor BT-R1. Here we show that binding of Cry toxin to BT-R1 provokes cell death by activating a previously undescribed signaling pathway involving stimulation of G protein (Gαs)and adenylyl cyclase, increased cAMP levels, and activation of protein kinase A. protein kinase A pathway ismanifested by sequential cytological changes that include membraneblebbing, appearance of ghost nuclei, cell swelling, and lysis.The discovery of a toxin-induced cell death pathway specifically linked to BT-R1 in insect cells should provide insights into how insects evolve resistance to Bt and into the development of new,safer insecticides.Induction of the adenylyl cyclase 3.请叙述白蚁肠道中的Chitinase基因有何运用潜力(5%) 4.请列举蜜蜂对於人类或科学上的五项贡献,并加以注解(10%) 例:蜂蜜,食物及医疗上的用途(不过如果这样写不算分) 5.请说明如何区分国内第一、二、三级保育动物,说明其列为保育的原因,并列出 保育类昆虫第一级1种、第二级5种(15分) 6.试比较生物指标及理化指标的优缺点,并举出昆虫应用在生物指标的实例(10%) 7.请简述饲养昆虫宠物的优点,并介少外来宠物昆虫有哪些潜在风险(10%) 8.何谓bio-inspiration?以"How aphids lose their marbles"来说明蚜虫如何 达到和莲叶的自洁效应之相同处,而把蜜露排出植物。并举出昆虫已商业运用於 仿生学的例子(15%) 9.请提供课程建议,请问本学期课程有哪些部分是可以安排多一点的内容,而哪些部 分是可以精简?或是相关课程建议以供改善(10%) --

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