大家好 目前看文献有看到将材料与CAM做贴附测试，因为不是 本科系所以不太懂这样做的目的，实验内容如下: In brief, fertilized Brown Leghorn eggs (Needle Farm) were incubated at 38C in a humidified forced flow incubator. All procedures were carried out under the Animals Scientific Procedures Act 1986. On day 3 of incubation, 2 ml of albumin was removed to detach the developing chorioallantoic membrane (CAM) from the shell; the eggs were then windowed and sealed with adhesive tape. On day 7 of incubation, the tape was removed and the CAM was slightly scratched with forceps to induce a small amount of bleeding. Small pieces (approximately 2x2 mm) of POSS-PCU(本篇使用的材料) seeded with or without hADSCs were placed on top of the scratched area of the CAM (6 eggs per group). The eggs werethen sealed with adhesive tape and returned to the incubator for additional 7 days of incubation. The scaffolds were then photographed in ovo at day 14 of incubation, before overnight fixation in 4% PFA. The scaffolds were then washed in PBS, embedded in OCT, sectioned and processed for immunocytochemistry as described above. 有疑问的问题如下: 1.把2ml的蛋白去除的目的?(第四行文字) 2.开孔的位置是否有固定的地方?(上网找资料有看没有懂orz) 3.测试之前为何要把CAM刮出血?(第八行文字) 最後还有个疑问，关於细胞固定，使用甲醇跟乙醇固定，是否有某种意义上 的差异?小弟的认知只有脱水 劳请版上乡民指导 谢谢 --

※ 发信站: 批踢踢实业坊(ptt.cc), 来自: 140.116.159.183 ※ 文章网址: https://webptt.com/cn.aspx?n=bbs/Biotech/M.1545301034.A.E45.html