作者vaio1213 (一整个无力)
看板Biotech
标题[求救] Invertrd invasion assays
时间Sat Jan 9 21:24:29 2010
我晓得Matrigel invasion assay的方法
但是Inverted invasion assay我一直看不懂
以下是paper上叙述的方法:
Matrigel (BD biosciences) or collagen (BD biosciences), supplemented with
25 μg/ml fibronectin (FN, Sigma) was polymerized in transwell inserts
(Corning) for 45 minutes at 37°. The inserts were inverted and 3-5x105
cells were seeded at the base of the insert. The inserts were placed in
serum free medium and the upper chamber was filled with 10% FCS and
25ng/ml EGF (Sigma) or 1U/ml TGFβ (Preprotech).
我不懂的是将insert反过来後
细胞要怎麽种在insert上?
然後insert又要放进serum free medium内
细胞不会再掉出来吗?
麻烦大家了 谢谢
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