The biology of Cancer; Robert A.Weinberg Ch7 TSG-synopsis p247 In the great majority of cases, both copies of a TSG must be inactivated before an incipient cancer cell enjoys any proliferative or survival advantages. Still, this rule is not a hard-and-fast obe. In some instances, such as the case of Nf1 gene, it appears that loss of one copy of a TSG already provides a measure of growth advantage of a cell. Mutant alleles of another TSG-p53 can create a partially mutant cell phenotype by actively interfering with the going functions of coexisting wilde-type allele. --- p248 The functional test has been made more rigorous by determining whether a candidate TSG, when expressed at normal, physiologic levels, halts the growth of a cancer cell lacking expression of this gene while leaving normal, wild- type cells from the same tissue unaffected. --- These consideration have led to an even stricker genetic definition of a TSG: a gene can be called a tumor supressor gene only if it undergoes LOH in many tumor cell genomes and if the resulting homozygous alleles bear clear and obvious inactivating mutations. --- 对了 假如你knockdown的是wt-p53..剩下30%的wt-p53还是足够执行功能XD --

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