作者tenyu (神的宠儿)
看板Biotech
标题[求救] 如果你在NEBcutter找的酵素切点...
时间Wed Aug 26 15:35:04 2009
因为要设计snp，刚开始学没有很久
在收寻限制酵素时，有一些序列填入後，点取酵素，她会出现以下几种信息
（1）Always blocked by CpG methylation.
(2)This site overlaps a CpG site: cGGCGCC , overlapping sites may block or
impair cleavage if methylated.
(3)This site overlaps a CpG site: GGACCg , overlapping sites will block cleavage
if methylated.
请问这代表这些区域容易甲基化，酵素切不动，所以不宜选取此酵素
是这个意思吗？
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