请问一下板上有在做老鼠的学长姐们 小弟念到一篇论文，用老鼠来做 MECP2基因缺失的实验。这篇文在材料方法中提到 All of the mice used in these experiments were generated by crossing heterozygous female Mecp2 Flox/+ mice that had been backcrossed to 129S6/SvEvTac for at least five generations to male mice on either a pure FVB/N background or male mice on a pure C57BL/6J background to generate isogenic F1 animals. Only male animals were used for all the experiments listed with WT littermate males serving as the controls. 我自己对这一段的理解是 他用 129S6 ，基因型 XX 的母鼠 和 FVBN、C57BL/6J 两种品系的公鼠去交配，变成 XX XY (此基因X-Link，X代表某基因被Knokout，X是正常性染色体) 生出子代的公鼠有两种，一种是 XY ，一种 XY XY 拿来做实验；XY当作 wild type control 但我的问题是，实验者怎麽知道这只老鼠是 XY 还是 XY ？ 想了很久，不得其解，来版上请学长姐不吝赐教 附上原文 http://www.badongo.com/file/15942038 -- 「我曾经听人说过，不管是什麽，当你不能够再拥有，你唯一可 以做的，就是令自己不要忘记。」 王家卫－东邪西毒 --

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