作者lenti (啄木鸟)
看板Biotech
标题Re: 请问有关lentivirus vector
时间Tue Mar 24 02:58:28 2009
※ 引述《allergy1115 (白天彼此明争暗斗)》之铭言：
: 最近确定我要改用lentivirus vector tet on/off system
: 找了invitrogen, 不太确定这家有这套系统吗,还是我眼残@@"
: 请高手帮忙解惑一下谢谢...!
: ps 除了Neo 是用G418在筛选细胞, 还有哪种gene(marker)
: 也是用G418在筛稳定clone的呢？
TronoLab 提供.
直接到Addgene 网站申请,
http://www.addgene.org/pgvec1
只需付运费.
pLVTHM
http://tronolab.epfl.ch/webdav/site/tronolab/shared/control/plvthm.pdf
pLV-tTRKRAB
http://tronolab.epfl.ch/webdav/site/tronolab/shared/control/plvttrkrab.pdf
pLV-tTRKRAB-Red
http://tronolab.epfl.ch/webdav/site/tronolab/shared/control/plvttrkrabred.pdf
The target cells need to be cotransduced with both pLVTHM and pLV-tTRKRAB-Red
to achieve conditional expression of siRNA. However pLVTHM alone can be used
for constitutive expression of siRNA.
Open Biosystem 探索科技代理
这家的lentivector 都不错, 但我没有用过Tet on/off system.
TRIPZ lentiviral inducible shRNAmir
for tightly regulatable RNAi
http://www.openbiosystems.com/RNAi/shRNAmirLibraries/TRIPZlentiviralinducibleshR/
Taiwan
GenDiscovery Biotechnology Inc
Phone: 886-2-8691-8491
Fax: 886-2-8691-8479
email:
[email protected]
http://gendiscovery.com.tw/index.html
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