作者WHATISGACKT ()
看板Biotech
标题[方法] DCFH-DA assay in 293T
时间Tue Jan 6 18:12:30 2009
因为最近某个实验要测ROS
可是reagent其实放了一阵子
想先test一下 DCFH-DA是否还可以使用?
here is the protocol
1. 293T without serum, 37 degree C, for 15 mins
2. add DCFH-DA 50 microM, 37 degree C, for 15 mins
3. trypsin digestion
4. resuspend in PBS
5. flowcytometry analysis
i was wondering if there is any step could be modified ?
thanks
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※ 编辑: WHATISGACKT 来自: 140.112.129.10 (01/06 18:13)
1F：推 paua:有加positive control组吗? 另外我记得应该1uM DCFHDA即可~ 01/07 11:59