作者aborwang (Abor)
看板Biotech
标题Re: [求救] Western Reprobe
时间Tue Sep 23 23:15:12 2008
1M Tris pH 6.8 --- 6.25 ml
10% SDS --- 20 ml
beta-mercaptoethanol --- 10 ml
=============================================
+ddH20 补到total 100 ml
在温度57~70度C中incubate with mild shaking 30 mins
然後1x TBS-T wash 3次，各10 mins
接着从Blocking开始做即可
NC membrane是可以stripping啦，不过会变的很脆弱，小心点work吧
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1F：推 hchs890843:我会洗到没有2-ME的味道为止 09/24 13:45