不好意思请问大家 这段的resuspend到底是在哪个地方呢 我查了原来灌洗的方法还是跟老师提供的paper叙述方法一样 麻烦大大提供意见 感恩 以下 Nasal Lavage Nasal lavage was performed as described earlier with some modifications (12). Briefly, 4.5 ml of prewarmed Hanks' balanced salt solution (HBSS) (37° C) was instilled through a heat-softened catheter into the nare. The subject held his or her chin down toward the chest and held the catheter in place by pinching the nares closed. The cartilaginous bridge of the nose was vibrated by a pediatric precursor while the fluid was refluxed three times. The lavage fluid was recovered and the procedure repeated in the opposite nare. The lavage fluid was placed immediately on ice until processing. Thereafter the lavage fluid sample was centrifuged, the cells were resuspended in 2 ml of supernatant of the lavage fluid, and the rest of the cell-free supernatant was aliquoted and frozen. 到底是 1.抽到剩下2cc resuspened 2.抽上清液2cc 下面沉淀物拿去2cc resuspend 3.抽上清液两cc resuspend(....) 因为有点不太了解字面上嚜意思 希望有德的大大可以解惑 感恩 A volume of 100 μl of resuspended cell suspension was used for cytospin; the remaining cell suspension was incubated for 20 h at 37° C, and centrifuged. The cells were frozen for Western blot analysis of iNOS. Nitrite production was measured in 100 μl of the supernatant, with the remainder being collected and frozen for later cytokine analysis. --

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