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标 题Re: [问题] Cloning:Enzyme digestion check
发信站清华生命科学 BBS (Sun Jul 1 14:39:01 2007)
转信站ptt!Group.NCTU!grouppost!Group.NCTU!nthuls
※ 引述《[email protected] (Live.myself)》之铭言：
> I am trying to clone a 750bp fragment into a vector.
> So far I have got several candidates after transformation.
> I purified the DNA by mini-prep
> I am wondering could I see the 750bp on DNA gel after enzyme check,
> Does anyone have experiment about similar size cloning, and be successful in
> the enzyme check?
> Thank you,
It depends on how the "enzyme check" experiment is designed.
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