看板Biotech
标 题Re: [问题] anti-GST的选购问题....
发信站清华生命科学 BBS (Fri May 4 21:46:02 2007)
转信站ptt!Group.NCTU!grouppost!Group.NCTU!nthuls
※ 引述《[email protected] (星期四去luna....)》之铭言：
> 由於订购了一组带有GST tag的fusion protein(Novus的)...
> 现在想定anti-GST monoclonal 的抗体....
> 可是看sigma的说明有点不太懂...
> Monoclonal anti-Glutathione-S-Transferase (GST)
> recognizes native as well as denatured-reduced forms of
> purified GST proteins, applying immunoblotting,1 dot
> blotting, and ELISA.The antibody is specific for GST
> from Schistosoma japonicum. The antibody does not
> recognize GST from rat, rabbit, porcine or bovine liver, or
> from human placenta, when tested by ELISA.
> 这意思是说......这只抗体会hi到Schistosoma japonicum内生性的GST,
> 而不会hi到鼠,兔......人的内生性GST吗..??
yes
> 我是要买来作western的....可是这抗体的说明没有reference(没有paper用过)
> 请问这抗体是不是不适用我的实验阿....还是有推荐好用的anti-GST??
> 拜托大家..p^^q..
This one is good.
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