作者lgirl (單身日記)
看板b884040XX
標題Re: 生化實驗
時間Wed Dec 12 23:46:22 2001
※ 引述《Filippo (小波)》之銘言：
: ※ 引述《Filippo (小波)》之銘言：
: : 都是一邊找NO一邊找PCR的結果
: : 真是器死我ㄌ
: real time~
: http://www.vu-wien.ac.at/i123/VIROKURS/RT-PCRkurs.html
: 一半德文一半英文
The method measures PCR product accumulation through a dual-labeled
fluorogenic probe (i.e., TaqMan Probe). This method provides very
accurate and reproducible quantitation of gene copies. Unlike other
quantitative PCR methods, real-time PCR does not require post-PCR
sample handling, preventing potential PCR product carry-over contamination
and resulting in much faster and higher throughput assays. The real-time PCR
method has a very large dynamic range of starting target molecule
determination (at least five orders of magnitude). Real-time quantitative
PCR is extremely accurate and less labor-intensive than current quantitative
PCR methods.
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