※ [本文轉錄自 NTU-Exam 看板] 作者: cuddi (找自己) 看板: NTU-Exam 標題: [試題] 94下 劉春櫻 分析化學二 第一次期中考 時間: Mon Apr 3 18:29:03 2006 課程名稱︰分析化學二 課程性質︰系定必修 課程教師︰劉春櫻 開課系所︰化學系 考試時間︰2006/3/30 8:20~10:00 試題 : 1.Define the following terms:(24%) a)isobestic point b)aptamer c)stroke shift d)quenching e)nebulizer f)antibody g)flow injection analysis h)chemiluminescence 2.What are the advantages and disadvantages of decresing monochromator slit width?(3%) 3.Describe how charge coupled device can be used as an extremely sensitive detector.(6%) 4.How to get the composition of a complex with the method of continous variation?(8%) 5.Explain how a laser generates light. List important properties of laser light.(8%) 6.How to measure the equilibrium constant with the Scatchard plot?(8%) 7.Describe the differences between the following and list any paticular advantages possessed by one over the other:(15%) a)phototubes and photomultiplier tubes b)conventional and diode-array spectrophotometers c)fluorescence immunoassay and time-resolved fluorescence immunoassay 8.(a)Explain the following figure in terms of each labeled words. (b)Disgussed how the property of fluorescent species dependent on the parameters of molecular structure, structure rigidity, temperature and solvent effects.(20%) 圖請參考 Quantitive chemical analysis/Daniel C. Harris/6ed p.421 fugure 18-13 9.Explain the following figure:(8%) 圖請參考 Quantitive chemical analysis/Daniel C. Harris/6ed p.433 上圖 10.Here is an immunoassay for explosive such as trinitrotoluene(TNT). 1)A column is prepared with covalently bound antibodies to TNT. Fluorescent-labeled TNT is passed through the column to saturate the antibodies with labeled TNT. The column is washed excess solvent until no fluorescent is detected at the outlet. 2)Air containing TNT vapors is drawn through a sampling device that concentrates traces of the gaseous vapors into distilled water. 3)Aliquots of water from the sample sre injected into the antibody column. The fluorescence intensity of the liquid exiting the column is proportional to the concentration of TNT in the water from the sampler over the range 20 to 1200 ng/ml. Draw pictures showing the state of the column in step 1 and 3 and explain how the detector works.(8%) --- --

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