課程名稱︰奈米光學影像和光譜 課程性質︰選修 課程教師︰陳淇、陳壁彰等 開課學院：理學院 開課系所︰物理系 考試日期（年月日）︰2016/04/29 考試時限（分鐘）：120 mins 試題 : Choose one of two questions from each lecture. (20x7=140pts in total) L1 (1) What is diffraction limit? How to reduce the size of focused spot as close to the diffraction limit as possible? (2) Compare the advantage and disadvantage of far-field and near-field superresolution imaging methods. L2 (1) Typical "extinction spectrum" contains both absorption and scattering loss. Proposal your way of measurement fot each component. (2) Illustrate the mechanism of fluorescence process and at least two possible competing channels to reduce the fluorescence quantum yield. L3 (1) What does STED stand for? What are the two major tricks to achieve superresolution with STED microscopy? (2) What does STORM stand for? What are the two major tricks to achieve superresolution with STORM? L4 (1) It is challenging for 3D live fluorescence imaging with subcellular spatial resolution, please explain the reasons? (2) What is the difference between epi-illumination and plane illumination configuration? What's the pros and cons for these two? L5 (1) What does CARS stand for? What are the advantages and limitations for CARS microscopy? (2) What is the order of magnitude difference for Raman, CARS, and fluorescent signals? And why? L6 (1) Obtain the permittivity expression using Drude model. (2) Describe the resonance of a plasmonic nanoparticle by Mie theory, Spring-mass model and Fabry-Perot model. L7 (1) Explain how a plasmonic Archimedes Spiral can serve as a bifunctional optical trap for focusing and rotation. (2) Explain how a slat-gap nanoantenna can enchance circular dichroism signal of chiral matter. -- 緬因貓是一種大貓貓 貓貓貓貓貓貓貓 --

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