課程名稱︰分子生物學 課程性質︰選修 課程教師︰張俊哲 丁詩同 余榮織(找不到字) 合開 開課學院：生農 開課系所︰昆蟲系 考試日期︰98/10/20 考試時限（分鐘）：兩節課 是否需發放獎勵金：是 試題 : 後面數字是配分 Molecular Biology Mid-term Examination 1. Write down the full spellings of DNA and RNA(in English; 4%) 2. Draw a basic scheme of "" nucleoside" and "nucleotide." 4 3. In the Hershey-Chase experiment, which components of the bacteriophage T3 are labelled with the radioactive isotopes, and what kind of isotopes were used? In addition, according to the findings of Hershey and Chase, what is the genetic material? 6 4. What was the key experimental data that helped Watson and Crick discover the structure of DNA? And, which form of the DNA double helix was shown in the experimental data? 4 5. Briefly describe the "Chargaff's rules." 4 6. Why most DNA binding protein bind the major grooves rather than the minor grooves? 2 7. Define the melting temperature of DNA (Tm). 2 8. Briefly explain why the supercoiled form of a plasmid DNA moves faster than the linearized form in an agarose gel during electrophoresis? 4 9. Define "nucleosome" and explain whether naked DNA or the DNA associated with the nucleosome is more accessible to the DNase digestion. 6 10. Illustrate a "phosphodiester bond/bridge" in a DNA stand and briefly explain why the polarity of a DNA strand is described as 5' to 3' or 3' to 5. 4 11. What are the five major type of RNA? 5 12. Define "molecular chaperones" and briefly describe their functions. 5 13. Briefly explain how a normal cellular prion(PrPc) is converted into a Scarpie prion protein(PrPsc)? 5 14. Describe the Meselsen and Stahl experiment and explain why it demostrates that DNA replication is semi-conservative. Note: please use illustration to hel your description. 10 15.Illustate and briefly describe the structure of a replication fork. Note: just illustrate the nucleic acid scheme. No proteins are required to show. 6 16. Briefly sescribe the roles of "clamp loader" and "sliding clamp" during DNA replication. 4 17. DNA synthesis is catalyzed by DNA polymerase. What are the two main functions of DNA polymerase? 4 18 Briefly describe the functions of : (1)DNA primase; (2)DNA helicase; (3)Single-strand DNA binding protein. 6 19. Illustrate and describe how Telomerase replicates the ends of chromosomes. 11 20. What are the achievements of 2009 Nobel prize in: (1) Chemistry and (2)Physiology or Medicine respectively? 4 --

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