第十張的題目有......這麼多!!!! =口= 大家一起集思廣益吧~ 加油嘿... 1. Q. Describe and give the results of an experiment that shows that polymerase I is located primarily in the nucleolus of the cell. 2. Q. Describe and give the results of an experiment that shows that polymerase III makes tRNA and 5s rRNA. 3. Q. Describe how epitope tagging can be used to purify Pol II from yeast in one step. 4. Q. Some preparations of Pol II show three different forms of the largest subunit. Give the names of these subunits and show their relative positions after SDS-PAGE. What are the differences among these subunits? Present evidence for these conclusions. 5. Q. What is the structure of the CTD of RPB1? 6. Q. Draw a rough diagram of the structure of yeast RNA Pol II. Show where the DNA lies, and provide another piece of evidence that supports this location for DNA. Also, show the location of this active site. 7. Q. How many Mg2+ ions are proposed to participate in catalysis at the active center of RNA polymerase? Why is one of these metal ions difficult to see in the crystal structure of yeast RNA pol II? 8. What is meant by the term “processive transcription?” What part of the Pol II structure ensures processivity? 9. What are the probable functions of the lid, rudder and zipper of Pol II? 10. What is the probable function of the bridge helix? What is the relationship of a-amanitin to this function? 11. Q. What are the E site and A site of RNA Pol II? What roles are they thought to play in nucleotide selection? 12. Q. what is the nature of the promoter located in the region encompassing the 37bp upstream of this start site? 13. Q. what is the nature of the promoter located in the region encompassing the 37bp upstream of this start site? 14. 問題: 也許是pol II而非polIII轉錄這基因? 15. Q. How do enhancers stimulate transcription? 16. Q. Is this really an enhancer? --

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