標題：A dictionary on microRNAs and their putative target pathways (有關microRNA的資訊) Christina Backes(1), Eckart Meese(2), Hans-Peter Lenhof(1) and Andreas Keller(3). (1)Center for Bioinformatics, Saarland University (2)Department of Human Genetics, Saarland University (3)The Biomarker Discovery Center Heidelberg, Germany Received December 31,2009.Revised February 24, 2010. Accepted February 27,2010. While in the last decade mRNA expression profiling was among the most popular research areas, over the past years the study of non-coding RNAs, especially microRNAs (miRNAs), has gained increasing interest. For almost 900 known human miRNAs hundreds of pretended targets are known. However, there is only limited knowledge about putative systemic effects of changes in the expression of miRNAs and their regulatory influence. We determined for each known miRNA the biochemical pathways in the KEGG and TRANSPATH database and the Gene Ontology categories that are enriched with respect to its target genes. We refer to these pathways and categories as target pathways of the corresponding miRNA. Investigating target pathways of miRNAs we found a strong relation to disease-related regulatory pathways, including mitogen-activated protein kinase (MAPK) signaling cascade, Transforming growth factor (TGF)-beta signaling pathway or the p53 network. Performing a sophisticated analysis of differentially expressed genes of 13 cancer data sets extracted from gene expression omnibus (GEO) showed that targets of specific miRNAs were significantly deregulated in these sets. The respective miRNA target analysis is also a novel part of our gene set analysis pipeline GeneTrail. Our study represents a comprehensive theoretical analysis of the relationship between miRNAs and their predicted target pathways. Our target pathways analysis provides a ‘miRNA-target pathway’ dictionary, which enables researchers to identify target pathways of differentially regulated miRNAs. ________________________________________ The authors wish it to be known that, in their opinion, the last two authors should be regarded as joint First Authors. ※ 編輯: biocommander 來自: 59.112.213.15 (04/21 12:38)