1. A、B兩種不同細菌之DNA中所含的adenine 鹼基各為40%、10%，請分析A、B細菌 genomic DNA中所含的4 種鹼基所佔的百分比為何？哪依各菌朱試教有可能生長在溫泉環 境中？ 2. 課本習題8 3. Heteroduplexes made up of one strand of DNA and one strand of RNA typically form an A-form helix. Please give an explanation that explains the preference of the heteroduplex for A-form and B-form helix. 4. Why would reducing the concentration of salts (lowering the ionic strength) in a solution of double-stranded DNA lower the melting point of the DNA? 5. Two molecules of double-stranded DNA are the same length (1000 base pairs), but differ in base composition. Molecule 1 contains 20% A+T; molecule 2 contains 60%A+T. Which molecule has a higher tm (melting point)? How many C residues are ther in the 60% A+T DNA molecule? (A) 2: 40 (B) 1: 200 (C) 2; 400 (D) 1; 400 (E) 2; 200 6. You wish to determine the relative degree of sequence similarity for DNA from different species of Galapagos finches (referred to as species A, B, C). You make hybrid duplexes of the DNA between A and B, A and C, and B and C. You measure the increase in the absorption of UV light with increasing temperature for each of the duplex solutions and obtain the following data: Hyvrid duplex tm A+B 80℃ A+C 87℃ B+C 83℃ (a) Which two species have a greater degree of similarity in their DNA sequences? Why? (b) What factors should be taken into account when interpreting these data? 7. 請畫出single strand 的詳細化學構造; DNA G-A-C-T-T，並畫出其互補之RNA化學構 造。 8. Please describe the effects of spontaneous deamination of C to U, or 5-methyl-C to T, on the next DNA replication? 9 .Read Assigned reading “ Your Genes, Your Choices “ Chapter 1 &2 ● (1)請用A4紙書寫。 (2)不用抄題。 (3)繳交時間：_ 老師說希望 禮拜三 五點以 前作業可以繳交到 她的辦公室 因此，請在 禮拜三 上生化課 《前》 統一繳交給 班代 甲班請交給 阿法 乙班請交給 彥廷 因為乙班三點還有課，所以可能需要麻煩 阿法 送往 醫學大樓 二樓 生化科辦公室 給 李妙蓉老師 謝謝大家的配合 --

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