qPCR NEWS - focus on integrated analysis of microRNA and mRNA expression ------------------------------------------------------------------------ Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is: * Data Analysis and BioInformatics in real-time qPCR - new sub-page =3D> http://integrated-analysis.gene-quantification.info * Follow ou new established "GeneQuan Daily NEWS" - a daily updated newsletter - http://Daily-News.gene-quantification.info * GenEx - a powerful tool For qPCR data analysis - download a free trial version - http://GenEx.gene-quantification.info ---------------------------------------------------------------------------= -- Integrated analysis of microRNA and mRNA expression http://integrated-analysis.gene-quantification.info Bioinformatics is a multidisciplinary approach to discribe, model and understand biological processes on basis of information on genes, transcripts (mRNA and microRNA), proteins and metabolism. It uses computers, data bases and algorithms to link all the information and translate it back into biology, physiology and pathophysiology. BioInformatics =3D> Database Management Systems, Data Mining, Sample Tracking, Information Management, Data Acquisition, Data Analysis, Statistics, Pattern Recognition & Classification, Simulation & Modeling Bioinformatics initially centered on sequence, genome, and transcript analysis but now the extensive use of microarrays, mass spectrometry, qPCR and RT-qPCR, RNA-Seq, has stimulated bioinformatic work in data acquisition, signal processing, and data mining. Also, simulation and modeling are becoming increasingly important areas of focus in bioinformatics which finally will lead to a new level of understanding the networks in the metabolism: Genomics, Transcriptomics, Splicomics, Proteomics, Metabolomics, Integrated analysis of microRNA and mRNA expression etc. Various papers will be presented explaining the integrated analysis of expressed mRNA and microRNA. Most of the shown publications are connected to web based data mining tools for free access. * MAGIA, a web-based tool for miRNA and Genes Integrated Analysis * The microRNA body map: dissecting microRNA function through integrative genomics * mirConnX: condition-specific mRNA-microRNA network integrator * miRGator: an integrated system for functional annotation of microRNAs * miRGator v2.0: an integrated system for functional investigation of microRNAs * Identification of microRNA-regulated gene networks by expression analysis of target genes * miRNAMap 2.0: genomic maps of microRNAs in metazoan genomes * miRecords: an integrated resource for microRNA-target interactions * miRTarBase: a database curates experimentally validated microRNA- target interactions * DIANA-microT Web server upgrade supports Fly and Worm miRNA target prediction and bibliographic miRNA to disease association * The DIANA-mirExTra web server: from gene expression data to microRNA function * MicroRNA and mRNA integrated analysis (MMIA): a web tool for examining biological functions of microRNA expression * mESAdb: microRNA expression and sequence analysis database * A novel computational framework for simultaneous integration of multiple types of genomic data to identify microRNA-gene regulatory modules * RNAhybrid: microRNA target prediction easy, fast and flexible * MirZ: an integrated microRNA expression atlas and target prediction resource * Identifying transcriptional start sites of human microRNAs based on high-throughput sequencing data * PolymiRTS Database 2.0: linking polymorphisms in microRNA target sites with human diseases and complex traits * miRNEST database: an integrative approach in microRNA search and annotation .... ... and much more =3D> http://integrated-analysis.gene-quantification.i= nfo ---------------------------------------------------------------------------= -- Follow ou new established "GeneQuan Daily NEWS" A daily updated newsletter around gene quantification and qPCR - http://Daily-News.gene-quantification.info ---------------------------------------------------------------------------= -- GenEx 5 - A Powerful Tool For qPCR Data Analysis Download a free trail version here =3D> http://GenEx.gene-quantification.in= fo GenEx is a popular software for qPCR data processing and analysis. Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to advanced cutting-edge data analysis. View our webpage =3D> http://GenEx.gene-quantification.info Basic data editing and management Arguably the most important part of qPCR experiments is to pre-process the raw data into shape for subsequent statistical analyses. The pre- processing steps need to be performed consistently in correct order and with confidence. GenEx Standard=92s streamlined and user-friendly interface ensures mistake-free data handling. Intuitive and powerful presentation tools allow professional illustrations of even the most complex experimental designs. Advanced cutting-edge data analysis When you need more advanced analyses GenEx Enterprise is the product for you. Powerful enough to demonstrate feasibility it often proves sufficient for most users demands. Current features include parametric and non-parametric statistical tests, Principal Component Analysis, and Artificial Neural Networks. New features are continuously added to GenEx with close attention to customers=92 needs. New features Sample handling and samples individual biology often contribute to confounding experimental variability. By using the new nested ANOVA feature in GenEx version 5 user will be able to evaluate variance contributions from each step in the experimental procedure. With a good knowledge of the variance contributions, an appropriate distribution of experimental replicates can be selected to minimize confounding variance and maximize the power of the experimental design! For experiments with complex features, such as for example multifactorial diseases, analytical relationships and classifications may not readily be available. The support vector machine feature in the new version of GenEx is so easy to use that it will make this advanced supervised classification method easily available to novice users, while providing access to advanced parameters for experts. Download a free trail version here =3D> http://GenEx.gene-quantification.in= fo ---------------------------------------------------------------------------= -- Please forward this qPCR NEWS http://api.addthis.com/oexchange/0.8/forward/email/offer?url=3Dhttp://qPCRn= ews.gene-quantification.info&title=3DJoin+our+monthly+newsletter+on&usernam= e=3DqPCR-NEWS&email_template=3D&lng=3Den-us to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages If this newsletter is not displayed correctly by your email client, please use following LINK http://qPCRnews.gene-quantification.info/ ---------------------------------------------------------------------------= -- The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl. Copyright =A92005-2012 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. Disclaimer & Copyrights are displayed on the homepage http://www.gene-quantification.info To subscribe or change your e-mail address in qPCR NEWS, and if you would like to receive future issues FREE of charge, please send an e- mail with the subject SUBSCRIBE mailto:newsletter@gene- quantification.info?subject=3DSUBSCRIBE