qPCR NEWS - November 2011 - focus on mirtrons --------------------------------------------------------------- Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is: * Introducing the mirtron - http://mirtron.gene-quantification.info * MIQE qPCR APP for IOS and Android is available - http://MIQE.gene-quantif= ication.info * MIQE guidelines - online translation service to CHINESE, JAPANESE, KOREAN and RUSSIAN If this newsletter is not displayed correctly by your email client, please use following http://qPCRnews.gene-quantification.info ---------------------------------------------------------------------------= -- Mirtrons were first identified in Drosophila melanogaster and Caenorhabditis elegans and are a type of microRNA that are located in the introns of the mRNA encoding genes. Mirtrons are alternative precursors for microRNA biogenesis. The short hairpin introns use splicing to bypass DROSHA cleavage, which is otherwise essential for the generation of canonical animal microRNAs. Mirtrons arise from the spliced-out introns and are known to function like classical microRNAs (miRs) and regulate gene expression, by either mRNA destabilisation, inhibition of the translation or target mRNA cleavage. =3D> http://mirtron.gene-quantification.info selected mirtron papers: MicroRNA - Introducing the mirtron Patrick Goymer, Nature Reviews Molecular Cell Biology 2007 (8): 597 And Now Introducing Mammalian Mirtrons Shih-Peng Chan and Frank J. Slack Department of Molecular, Cellular and Developmental Biology, Yale University, USA, Developmental Cell 13 (2007): 605-607 Mammalian mirtron genes Berezikov E, Chung WJ, Willis J, Cuppen E, Lai EC., Hubrecht Institute, Utrecht, The Netherlands, Mol Cell. 2007 28(2): 328-336. Mirtrons - microRNA biogenesis via splicing Jakub O. Westholm, Eric C. Lai Corresponding Author Contact Information, E-mail The Corresponding Author Department of Developmental Biology, Sloan-Kettering Institute, 1275 York Ave, Box 252, NY 10065, USA, Biochimie 93(11): 1897-1904 Intronic microRNA precursors that bypass Drosha processing J. Graham Ruby, Calvin H. Jan, & David P. Bartel Nature Letters Vol 448:5 2007 The biogenesis and characterization of mammalian microRNAs of mirtron origin Sibley CR, Seow Y, Saayman S, Dijkstra KK, El Andaloussi S, Weinberg MS, Wood MJ. Nucleic Acids Res. 2011 Computational and experimental identification of mirtrons in Drosophila melanogaster and Caenorhabditis elegans Chung WJ, Agius P, Westholm JO, Chen M, Okamura K, Robine N, Leslie CS, Lai EC. Department of Developmental Biology, Sloan-Kettering Institute, Rockefeller Research Laboratories, New York, USA, Genome Res. 2011 21(2): 286-300 miRNAs in human cancer Farazi TA, Spitzer JI, Morozov P, Tuschl T. Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, New York, NY 10065, USA, J Pathol. 2011 223(2): 102-115 The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila Okamura K, Hagen JW, Duan H, Tyler DM, Lai EC. Memorial Sloan-Kettering Cancer Center, Department of Developmental Biology, New York, USA, Cell. 2007 130(1): 89-100 MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila Flynt AS, Greimann JC, Chung WJ, Lima CD, Lai EC. Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, Box 252, New York, NY 10065, USA, Mol Cell. 2010 38(6): 900-907 Intronic noncoding RNAs and splicing Brown JW, Marshall DF, Echeverria M. Plant Sciences Division, University of Dundee at the Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, UK, Trends Plant Sci. 2008 13(7): 335-342 and much more papers are available here =3D> http://mirtron.gene-quantifica= tion.info ---------------------------------------------------------------------------= -- MIQE_qPCR APP for - iOS Universal & Android version Get help from a special team of experts in qPCR while on the move. MIQE - qPCR helps you in reviewing scientific works and checking your own experiments, when qPCR is involved. Check your project's compliance to MIQE in minutes, have all required references to hand, and follow qPCR events and news..... http://www.gene-quantification.de/miqe-qpcr-app-slide-show.pdf Over 2,400 downloads =3D> http://itunes.apple.com/app/miqe-qpcr/id423650002?mt=3D8 MIQE_qPCR Android version - Now available in Nov 2011 =3D> https://market.android.com/details?id=3Dcom.biorad.miqeqPCR ---------------------------------------------------------------------------= -- Please forward this qPCR NEWS http://api.addthis.com/oexchange/0.8/forward/email/offer?url=3Dhttp://qPCRn= ews.gene-quantification.info&title=3DJoin+our+monthly+newsletter+on&usernam= e=3DqPCR-NEWS&email_template=3D&lng=3Den-us to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages If this newsletter is not displayed correctly by your email client, please use following LINK http://qpcrnews.gene-quantification.info/ ---------------------------------------------------------------------------= -- The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl. Copyright =A9 2005 - 2011 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. 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