qPCR Newsletter September 2008 Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is: - microRNA - new links and animations - microRNA - updated microRNA application papers - worldwide qPCR application workshops - qPCR Symposium USA in November 2008 - register now ! - 1st announcement qPCR 2009 Event =3D> http://www.qPCR2009.net ---------------------------------------------------------------------------= ----- New http://www.Gene-Quantification.ASIA pages online translation to CHINESE =3D> http://chinese.gene-quantification.asia/ JAPANESE =3D> http://japanese.gene-quantification.asia/ KOREAN =3D> http://korean.gene-quantification.asia/ RUSSIAN =3D> http://russian.gene-quantification.asia/ ---------------------------------------------------------------------------= ----- Links on http://microrna.gene-quantification.info/ microRNA Definition non-coding RNAs / DataBase micro RNA talks and microRNA posters Nature Genetics 38, S1 (2006) - The microRevolution miRNA Research Education Research Center Human MicroRNA targets miRU - Plant microRNA Potential Target Finder Cancer genomics: Small RNAs with big impacts miRU: an automated plant miRNA target prediction server miRBase - the home of microRNA data miRBase: microRNA sequences, targets and gene nomenclature The microRNA Registry A uniform system for microRNA annotation TRC - the RNAi Consortium shRNA Library Algorithms & Thermodynamics for Nucleic Acid Folding, Hybridization & Melting Profile Prediction TUTORIAL: Check out an illustrated tutorial on miRNA (by Ambion) ---------------------------------------------------------------------------= ----- A lot of new application papers using microRNA quantitative real-time RT-PCR http://microRNA.gene-quantification.info Strategies to determine the biological function of microRNAs. Genomics of microRNA. Tracing microRNA patterns in mice. Target mimics modulate miRNAs. Target mimicry provides a new mechanism for regulation of microRNA activity. Lessons from Nature: microRNA-based shRNA libraries. Identification of Differentially Expressed MicroRNAs by Microarray: A Possible Role for MicroRNA Genes in Pituitary Adenomas. RNA polymerase III transcribes human microRNAs. Real-time expression profiling of microRNA precursors in human cancer cell lines. Multiplexing RT-PCR for the detection of multiple miRNA species in small samples. An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. MicroRNA expression profiling using LNA-modified probes in a liquid- phase bead-based array. miRCURY=99 LNA research tools for microRNA. Editorial - The microRevolution. Glimpses of a Tiny RNA World. Functions of microRNAs and related small RNAs in plants. Evidence that microRNAs are associated with translating messenger RNAs in human cells. How microRNAs control cell division, differentiation and death. microRNA target predictions in animals. Human let-7a miRNA blocks protein production on actively translating polyribosomes. Computational identification of microRNA targets. Intronic microRNA precursors that bypass Drosha processing. MicroInspector: a web tool for detection of miRNA binding sites in an RNA sequence. Target labelling for the detection and profiling of microRNAs expressed in CNS tissue using microarrays. A brain-specific microRNA regulates dendritic spine development. ---------------------------------------------------------------------------= ----- Update of the Webinar page A lot of interesting TALKs, WEBINARs, SLIDE SHOWs, and PODCASTs from various speakers, biotec companies, qPCR Events, and international journals (Nature and Science) are FREE for download. Have a look and you will definitely something interesting for your scientific work ! http://webinar.gene-quantification.info/ ---------------------------------------------------------------------------= ----- With the new qPCR INFO PORTAL and all the presented tools we will help you with to find the right information about qPCR and related topics in Molecular Biology in the literature and in the World Wide Web. =3D> Papers / Protocols / Methods / Databases / Alets / Feeds / Books / Forums / E-mail / Directory http://infoportal.gene-quantification.info/ ---------------------------------------------------------------------------= ----- Upcoming Events World-wide academic and commercial qPCR Events http://events.gene-quantification.info/ Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars, qPCR Education Program, ...etc.. Please submit your qPCR event here =3D> events@gene- quantification.info ---------------------------------------------------------------------------= ----- qPCR SYMPOSIUM BENELUX The prominent and still growing place taken by real-time quantitative PCR in applied and fundamental research and clinical diagnostics almost appears obvious. However, it is clear that contributions made by various scientists and companies in the field during the last decade rendered this technology useful and affordable for many users. More info =3D> http://www.gene-quantification.de/meetings.html#benelux Importantly, the qPCR domain is still in constant evolution, making it sometimes hard to stay informed about new methodological approaches or original studies using the real-time PCR. Therefore, we have scheduled a one day "Benelux qPCR Symposium" on October 6th 2008, giving the opportunity to the scientific community to get informed and discuss various aspects of real-time PCR (including but not limited to new applications, assay optimization and validation, new technologies, etc.). Scientific talks, posters sessions and industrial booths will be at the menu. Download poster =3D> http://www.gene-quantification.de/qpcr_benelux_poster= ..pdf ---------------------------------------------------------------------------= ----- qPCR Symposium USA 10. - 13. November 2008 Clarion Hotel San Francisco Airport, Millbrae, CA , USA More info =3D> http://www.gene-quantification.de/meetings.html#qpcr_usa - High throughput platforms: High throughput applications, real-time RT-PCR arrays, digital PCR - Forthcoming technologies: Immuno PCR, Methylation sensitive PCR, SNP analysis, High resolution melt, microRNA detection, - Multiplex technologies - Single-cell qPCR: Pre-amplification techniques, sub-cellular PCR, Expression heterogeneity, laser microdissection, FACS sorting, Enrichment of rare cells - Multimarker diagnostics: Disease markers, Tissue specific markers, Cancer markers, Stem cells, Differentiation markers, Cancer stem cells - Real-time PCR Expression Profiling: multivariate and multiway expression profiling, temporal expression profiling, spatiotemporal maps - Pre-analytical Steps: Sampling technologies, Extraction methods, Reverse Transcription, Quality Control, Standards, Standard Operating Procedures, Interlaboratory Exercises - Normalization & Standardization: Normalization strategies, Reference genes, Spikes, Standard curves, multiplexing, inter-run calibrators, quantification strategies, mRNA degradation - Data management and data treatment: software applications, data mining, data visualization, biostatistics, multivariate statistics ---------------------------------------------------------------------------= ----- qPCR 2009 Event 1st annoncement of the qPCR 2009 Event in Freising Weihenstephan http://www.qPCR2009.net Download flyer =3D> http://www.bioeps.com/qpcr2009/qPCR-2009-1st-announcem= ent.pdf ---------------------------------------------------------------------------= ----- qPCR WORKSHOP TATAA Biocenter Germany - qPCR Application workshops At the TATAA Biocenter Germany we offer qPCR application workshops, the 3-day Core Module and a 2-day Biostatistics Module. qPCR courses are held in regularly in G=F6teborg, Sweden, in English and in Freising- Weihenstephan, Germany, in German and English, and in Prague, Czech Republic in English and Czech. Depending on the occasion the workshop language and the different prices may apply. Further customized workshops and specialized trainings will be held as well across Europe and world-wide. TATAA Biocenter Germany courses are held in cooperation with the Institute of Physiology, located at the Technical University of Munich, in Freising-Weihenstephan, near Munich, very close to the Munich Airport (MUC). For more information and to register for the qPCR application workshops, please see our web page: http://tataa.gene-quantification.info/ Course Occasions summer and autumn 2008: 13-17 Oct Prague RNA Isolation + qPCR Core Module + HRM 13-17 Oct Freising Germany qPCR Core Module + Biostatistics (Kurs wird in DEUTSCH gehalten, German language) 27-31 Oct G=F6teborg qPCR Core Module + HRM + Biostatistics 17-21 Nov Prague qPCR Core Module + Practical Biostatistics 24-28 Nov Freising Germany qPCR Core Module + Biostatistics (English language) 1-5 Dec G=F6teborg qPCR Core Module + Biostatistics 15-19 Dec Prague RNA Isolation + Expression Profiling and Data Analysis Download list of TATAA courses in autumn and fall 2008 Please register here =3D> http://www.tataa.com/Courses/Courses.html ---------------------------------------------------------------------------= ----- Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages http://www.gene-quantification.info ---------------------------------------------------------------------------= ----- If this newsletter is not displayed correctly by your email client, please use following link: http://qpcrnews.gene-quantification.info/ The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl. Copyright =A9 2005 - 2008 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. 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